Sybr Green Dna Gel Stain

Sybr green i nucleic acid gel stain is one of the most sensitive stains available for detecting double stranded dna dsdna in agarose and polyacrylamide gels.

Sybr green dna gel stain. Sybr safe in dmso freezes at low temperatures. Gelgreen is far more sensitive than sybr safe. Sybr safe dna gel stain 2 before starting storage handling and disposal you may store the sybr safe dna gel stain at any temperature between 2 c to 25 c. Repeated freeze thawing has minimal impact on product performance.

Because sybr green i has greater sensitivity for dsdna it is especially useful for assays where the presence of contaminating rna or ssdna might obscure results. 5 matches found for sybr safe dna gel stain advanced search structure search sort by relevance name name base name base name formula weight formula weight. Sybr green i is also a very sensitive stain for oligonucleotides allowing for detection of as little as 1 2 ng of a synthetic 24 mer on a 5 polyacrylamide gel which is 50 100 times greater. Sybr safe dna gel stain in 0 5x tbe is indistinguishable from 0 5x tbe alone in terms of organic pollutant content both samples tested negative for the presence of an extensive array of organic compounds table 3.

The stain preferentially binds to double. Sybr safe dna gel stain hazardous waste analysis. Sybr safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide eb that can be used with either blue light or uv excitation. Sybr safe dna gel stain is a highly sensitive stain for visualization of dna in agarose or acrylamide gels.

Unlike sybr dyes which are known to be unstable gelgreen is very stable both hydrolytically and thermally. Gelgreen is compatible 254 nm uv transilluminator and can be imaged using a sybr green or gelstar filter. Sybr safe dna gel stain is a highly sensitive stain for visualization of dna or rna in agarose or acrylamide gels. Sybr safe dna gel stain 2 before you begin handling and disposal sybr safe dna gel stain showed no or very low mutagenic activity when tested by an inde pendent licensed testing laboratory and this stain is not classified as hazardous waste under u s.

Sybr green i stain has been shown to be much less mutagenic than ethidium bromide in ames tests. Reduce your exposure to highly mutagenic. Sybr green i sg is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology the sybr family of dyes is produced by molecular probes inc now owned by thermo fisher scientific sybr green i binds to dna the resulting dna dye complex best absorbs 497 nanometer blue light λ max 497 nm and emits green light λ max 520 nm. Sybr green ii is recommended for ss dna and rna.

Therefore the product must be completely thawed and mixed before using.