Sybr Green Gel Stain

The stain preferentially binds to double.

Sybr green gel stain. Sybr green i sg is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology the sybr family of dyes is produced by molecular probes inc now owned by thermo fisher scientific sybr green i binds to dna the resulting dna dye complex best absorbs 497 nanometer blue light λ max 497 nm and emits green light λ max 520 nm. Sybr safe dna gel stain does not differ significantly from 0 5x tbe buffer when tested according to national pollutant discharge elimination system npdes guidelines table 2. Plus when used with blue light illumination sybr safe stain has less background fluorescence than ethidium bromide stained gels illuminated with uv light. Because sybr green i has greater sensitivity for dsdna it is especially useful for assays where the presence of contaminating rna or ssdna might obscure results.

Sybr green i is also a very sensitive stain for oligonucleotides allowing for detection of as little as 1 2 ng of a synthetic 24 mer on a 5 polyacrylamide gel which is 50 100 times greater sensitivity than obtained with ethidium bromide. Sybr green i nucleic acid gel stain is one of the most sensitive stains available for detecting double stranded dna dsdna in agarose and polyacrylamide gels. Sybr safe dna gel stain is not classified as corrosive ignitable or reactive under the guidelines of the environmental protection agency epa table 1.