Sybr Green Master Mix

Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Powerup sybr green master mix contains proprietary dual lock taq dna polymerase enzyme which uses a combination of two hot start mechanisms to control its activity.

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Sybr Green Sybr Green I Sybr Green Qpcr Sybr

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Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr.

Sybr green master mix. The azuraquant green fast qpcr mix is a ready to use 2x master mix for use in real time quantitative pcr assays in which intercalating dye based detection provides the option of a post amplification melt profile. Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume. Sybr green master mix advantages. Applied biosystems amplitaq gold dna polymerase ld is a hot.

Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa. Sybr green pcr master mix in a 1 ml vial. Find the best master mix by application. Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows.

The lightcycler 480 sybr green i master is a one component hot start reaction mix for pcr. As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. Providing tight control over taq activation this dual hot start approach helps prevent undesirable early activity of the polymerase at low temperatures that can lead to non specific amplification. The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr.

Power sybr green pcr master mix provides greater flexibility reduces experimental design time and requires fewer reagents. During each phase of dna synthesis the sybr green i dye which is included. Faststart universal sybr green master rox is a ready to use hot start reaction mix for qpcr and rt qpcr on all real time pcr systems requiring normalization with rox. Sybr green dye is a fluorescent double stranded dna dsdna binding dye that is used to track the progress of dna amplification in real time pcr experiments.

General description faststart universal sybr green master rox. Azuraquant green and probe fast qpcr master mixes for real time pcr real time pcr primer enzymes. It contains faststart taq dna polymerase and dna double strand specific sybr green i dye for product detection and characterization. Sybr green i is a dna double strand specific dye.