Sybr Green Pcr Master Mix

Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume.

Sybr green pcr master mix. Sybr select master mix is a superior and cost effective solution for your real time pcr applications. Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr. Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows. 2 it is also compatible with the bio rad iq 5 and cfx systems roche lightcycler 480 and agilent mx3005p systems.

Sybr green master mix advantages. The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr. Find the best master mix by application. Sybr green dye is a fluorescent double stranded dna dsdna binding dye that is used to track the progress of dna amplification in real time pcr experiments.

It contains faststart taq dna polymerase and dna double strand specific sybr green i dye for product detection and characterization. Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. For optimal results recommended primer concentrations should. Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis.

Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Sybr green pcr master mix in a 1 ml vial. Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa. As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step.