Sybr Green Qpcr

Standard curve r 2 0 999 β actin efficiency 92 6 slope.

Sybr green qpcr. Itaq universal sybr green supermix provides reliable gene expression data. Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. Itaq universal sybr green supermix produced greater than 90 efficiency over 6 orders of linear dynamic range. Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr.

The human β actin gene was amplified from hela cdna 100 ng 100 fg using a cfx96 real time pcr detection system. Sybr green qpcr supermixes are compatible with most protocols for qpcr. Sybr green pcr master mix and sybr green rt pcr reagents kit user guide 9 1 product information purpose of the kit the sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. In addition sybr green qpcr supermixes are available with either antibody mediated hot start taq or sso7d fusion polymerase which provides increased stability for the polymerase template complex enhancing performance.

Sybr green qpcr supermixes. The quantifast sybr green rt pcr kit delivers highly specific and sensitive results outperforming other real time rt pcr kits used in fast cycling mode see figure specific one step rt pcr rt pcr run times are reduced by up to 60 see figure significantly reduced pcr times allowing you to get results faster you can also greatly increase your sample throughput or efficiently share a. Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases. The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr.

In routine pcr the critical result is the final quantity of amplicon generated after the process. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. The miscript sybr green pcr kit is an integral component of the miscript pcr system for mirna detection and quantification. Real time or quantitative pcr and rt pcr use the linearity of dna amplification to determine.

With the development of thermal cyclers incorporating fluorescent detection pcr has a new innovative application. The kit is based on the proven technology of quantitect sybr green kits. Find the best master mix by application. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region.

Direct detection of pcr product is monitored by measuring the increase in.